Human Interleukin 13 ELISA Kit

Offered by Antibodies.com within the Assays and Kits category, this is a sandwich ELISA (sELISA) for the in vitro quantitative determination of human Interleukin 13 (IL-13). The assay is reactive with human samples and is compatible with serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids, using a pre-coated 96-well microplate arranged as 12 x 8 well strips and read on a colorimetric microplate reader at 450 nm.

Quantitative performance is characterised by a product range of 15.6-1000 pg/ml and a sensitivity of 7.1 pg/ml, with a total assay time of 4 hours 30 minutes. The protocol uses an immobilised anti-IL-13 capture antibody, a Biotinylated Anti-IL-13 detection antibody and an HRP-Avidin conjugate, with TMB substrate converted to a yellow product whose absorbance is proportional to the amount of IL-13 captured in each well.

Manufacturer-reported average recovery values are 91% for serum, 96% for EDTA plasma and 95% for heparin plasma, with linearity ranges provided across 1:2, 1:4, 1:8 and 1:16 sample dilutions. Kit components include a pre-coated microplate, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, stored at +4°C or -20°C as instructed and not used past the stated expiration date.