Human Interleukin-12 ELISA Kit

Quantitative detection of human Interleukin-12 p70 in research samples is achieved through a 192-well plate kit from Antibodies.com, useful for investigations of Th1 differentiation, IFN-gamma regulation, and innate immune signaling pathways.

IL-12 is a pro-inflammatory heterodimeric cytokine composed of p35 and p40 subunits, secreted by activated dendritic cells, macrophages, and B lymphocytes. It drives naive CD4+ T cell commitment toward the Th1 lineage and stimulates natural killer cell cytotoxicity, making it a key analyte in infectious disease research, vaccine immunology, and autoimmune disorder studies.

The kit includes pre-coated plate strips, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated recombinant standards, and assay buffer sufficient for 192 determinations. It is intended for research use only and is not validated for diagnostic procedures. Reconstituted standards and undiluted specimens should be handled per the included protocol to preserve epitope integrity, and aliquots of working standards should be prepared before first use.

Each kit ships refrigerated; frozen components should be brought to room temperature before opening to prevent condensation. Researchers measuring IL-12 as part of inflammation or cell culture studies will find this format a practical tool for individual-sample testing and higher-throughput screening of cytokine responses.