Human Interleukin 11 ELISA Kit

From Antibodies.com, this Interleukin 11 ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the in vitro quantitative determination of human IL-11 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids [S1]. The kit is listed under the Assays and Kits product category and is reactive with human samples [C0, S1].

The assay employs a pre-coated microplate (12 x 8 well strips) onto which an antibody specific for IL-11 has been immobilised [S1]. Standards and test samples are added to the wells, followed by a Biotinylated Anti-IL-11 Antibody, an HRP-Avidin conjugate, and a TMB substrate solution; the enzymatic reaction is terminated with acidic stop solution and the absorbance is read at 450 nm in a colorimetric microplate reader [S1]. The stated measuring range is 15.6-1000 pg/ml with a sensitivity of 5.8 pg/ml, and the total assay time is 4h 30m [S1]. Kit components are stored at +4°C or -20°C as instructed and should not be used past the expiration date [S1].

Recovery has been validated across sample types: serum (n=5) 83-100% with an average of 91%, EDTA plasma (n=5) 90-103% with an average of 96%, and heparin plasma (n=5) 89-102% with an average of 95% [S1]. Linearity has been demonstrated at dilutions of 1:2, 1:4, 1:8 and 1:16, with reported ranges of 79-110% in serum, 73-109% in EDTA plasma, and 77-110% in heparin plasma [S1].