Human Interleukin 10 Receptor Alpha ELISA Kit

A sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of human Interleukin 10 Receptor Alpha in tissue homogenates, cell lysates and other biological fluids. The plate is pre-coated with a specific capture antibody and the assay uses a biotinylated anti-IL-10RA detection antibody with HRP-avidin, TMB substrate and a colourimetric readout at 450 nm on a microplate reader. The manufacturer reports an assay range of 31.2-2000 pg/ml and a sensitivity of <13.8 pg/ml, while a third-party listing gives a detection range of 31.25-2000 pg/ml and a sensitivity of 13.8 pg/ml.

Standard protocol runs in 4 hours 30 minutes on a pre-coated 96-well microplate (12 x 8 well strips). Spike-and-recovery validation gave averages of 91% (range 80%-102%, n=5) in serum, 91% (81%-101%, n=5) in EDTA plasma and 84% (80%-89%, n=5) in heparin plasma. Third-party product information lists a rabbit host antibody of IgG isotype and describes the kit as supplied in liquid form in PBS with 0.02% sodium azide and 50% glycerol at pH 7.3.

Components are stored at +4°C or -20°C as instructed, and the supplier recommends avoiding freeze/thaw cycles. The product is sold for laboratory research use only and is not intended for human or animal therapeutic or diagnostic applications.