Human Interleukin 1 Receptor Accessory Protein Like Protein 2 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is intended for the in vitro quantitative determination of human IL-1RAPL2 (Interleukin 1 Receptor Accessory Protein Like Protein 2) in tissue homogenates and other biological fluids. The assay is performed on a pre-coated 96-well microplate arranged as 12 x 8 well strips.

Quantitative performance includes a sensitivity of <0.054 ng/ml and an assay range of 0.156-10 ng/ml, with a total run time of 4h 30m. Detection is colourimetric, read at 450 nm on a microplate reader using TMB substrate and an acidic stop solution. Kit components should be stored at +4°C or -20°C as instructed.

Validation data for serum, EDTA plasma and heparin plasma samples (n=5 each) show mean recoveries of 91%, 91% and 84% respectively, with linearity demonstrated across serial dilutions of 1:2, 1:4, 1:8 and 1:16.