Human Interleukin 1 Receptor Accessory Protein ELISA Kit

Antibodies.com offers a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit designed for the in vitro quantitative determination of human Interleukin 1 Receptor Accessory Protein (IL-1RAcP) in serum, plasma, tissue homogenates or other biological fluids. The assay is intended for human samples and is built around a pre-coated microplate platform.

The kit uses a sandwich ELISA format with a colorimetric detection read at 450 nm on a microplate reader. Reported sensitivity is <0.065 ng/ml, with an assay range of 0.156-10 ng/ml. The total assay time is 4h 30m.

Recovery performance across sample types is reported: Serum (n=5) 80% - 102% (average 91%), EDTA Plasma (n=5) 81% - 101% (average 91%), and Heparin Plasma (n=5) 80% - 89% (average 84%). Dilution linearity data are also reported for serum, EDTA plasma, and heparin plasma at 1:2, 1:4, 1:8, and 1:16 dilutions. Kit components include a pre-coated 96-well microplate (12 x 8 well strips), lyophilized standard, detection solutions, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C as instructed.