Human Interleukin 1 Epsilon ELISA Kit

A sandwich ELISA kit from Antibodies.com designed for the quantitative measurement of a human interleukin-1 family protein in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The catalogue entry lists the target as Interleukin 1 Epsilon, while the manufacturer datasheet identifies the analyte as IL-36 alpha/IL-1F6. Reactivity is specified as human, and the application is sandwich ELISA.

According to the manufacturer datasheet, the assay uses a pre-coated microplate with an immobilised capture antibody, a biotinylated anti-analyte detection antibody, HRP-Avidin conjugate and TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader. Reported sensitivity is 5.1 pg/ml, the standard curve range is 15.6-1000 pg/ml, and the total assay time is 4 hours 30 minutes. Average recovery values reported at n=5 (undiluted) are 91% for serum (range 83-100%), 96% for EDTA plasma (range 90-103%) and 95% for heparin plasma (range 89-102%).

Dilution linearity data are provided for serum, EDTA plasma and heparin plasma (each n=5) at 1:2, 1:4, 1:8 and 1:16 dilutions. Kit components include a lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers. Components are stored at +4°C or -20°C as instructed.