Human Intercellular Adhesion Molecule 1 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of human Intercellular Adhesion Molecule 1 (ICAM1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids [S1]. The assay uses an ICAM1-specific antibody pre-coated onto a microtiter plate, with detection via a biotinylated anti-ICAM1 antibody, HRP-avidin conjugate, and TMB substrate; the enzymatic reaction is stopped with acidic stop solution and absorbance is read at 450 nm on a colorimetric microplate reader [S1].

The product range is 78.1-5000 pg/ml with a sensitivity of <31 pg/ml [S1]. Kit components are stored at +4°C or -20°C as instructed, and the kit has been cited in 1 publication [S1].