Human Integrin Beta 6 ELISA Kit

Manufactured for quantitative in vitro measurement of human Integrin Beta 6 (ITGB6), this sandwich ELISA from Antibodies.com is intended for use with tissue homogenates, cell lysates, and other biological fluids. The assay is run on a pre-coated 96-well microplate and read colorimetrically at 450 nm using a standard microplate reader, with an overall assay time of 4 hours 30 minutes.

Reported performance characteristics include a sensitivity of less than 0.062 ng/ml and an assay range of 0.156–10 ng/ml. Recovery has been evaluated in serum (average 91%; range 83–99%, n=5), EDTA plasma (average 89%; range 80–99%, n=5), and heparin plasma (average 90%; range 82–99%, n=5). Dilution linearity has been assessed across 1:2, 1:4, 1:8, and 1:16 dilutions for serum, EDTA plasma, and heparin plasma samples. Storage of kit components at +4°C or -20°C is recommended, and the product should not be used past its expiration date.

The assay uses an HRP–avidin/biotinylated detection antibody system with TMB substrate; absorbance is read at 450 nm and the concentration of ITGB6 is calculated against a standard curve generated from a 1:2 serial dilution series.