Human Insulin Like Growth Factor Binding Protein 7 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human IGFBP7 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The plate is pre-coated with an antibody specific for IGFBP7; bound analyte is detected with a biotinylated anti-IGFBP7 antibody, an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The stated product range is 0.156–10 ng/ml with a sensitivity of 0.059 ng/ml. Total assay time is 4h 30m and components are stored at +4°C or −20°C as instructed. Reported recovery is 80–102% (average 91%) in serum (n=5), 81–101% (average 91%) in EDTA plasma (n=5), and 80–89% (average 84%) in heparin plasma (n=5). Linearity has been assessed across dilutions from 1:2 to 1:16 in the same sample types.