Human Insulin Degrading Enzyme ELISA Kit

Antibodies.com supplies this sandwich ELISA for the in vitro quantitative determination of human Insulin Degrading Enzyme (IDE) in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) with a biotinylated anti-IDE detection antibody and an HRP-avidin conjugate, developed with TMB substrate and read at 450 nm on a colorimetric microplate reader.

Performance figures from the manufacturer include a standard curve range of 62.5-4000 pg/ml and a sensitivity of 22.7 pg/ml, with a total assay time of approximately 4h 30m. Reported spike-and-recovery ranges are 80%-102% for serum (average 91%, n=5), 81%-100% for EDTA plasma (average 90%, n=5) and 80%-89% for heparin plasma (average 84%, n=5). Dilution linearity across 1:2 to 1:16 spanned 87-107% for serum, 79-108% for EDTA plasma and 82-105% for heparin plasma (each n=5).

Supplied components include a pre-coated 96-well microplate, lyophilized standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers. Components should be stored at +4°C or -20°C as instructed and the kit must not be used past the stated expiration date.