Human Immune Receptor Expressed On Myeloid Cells 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human CD300 in tissue homogenates, cell lysates or other biological fluids. It employs a pre-coated microplate and uses a biotinylated anti-CD300 detection antibody with HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader.

The stated assay range is 0.156–10 ng/ml with a sensitivity of <0.062 ng/ml, and the total assay time is 4h 30m. Reported average recovery is 91% for serum, 90% for EDTA plasma and 84% for heparin plasma, with dilution linearity data provided for serum, EDTA plasma and heparin plasma across multiple dilutions.

Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers. Components are stored between +4°C and -20°C as instructed and should not be used past the expiration date.