Human IL-17A/F ELSIA Kit

The Human IL-17A/F ELISA Kit from Antibodies.com enables quantitative detection of the biologically active IL-17A/F heterodimer, a cytokine complex co-secreted by Th17 cells that signals through the IL-17 receptor and drives mucosal inflammation, neutrophil recruitment, and autoimmunity. Measuring the heterodimer rather than either homodimer alone is relevant in psoriasis, rheumatoid arthritis, inflammatory bowel disease, and severe asthma research, where Th17 effector responses are commonly interrogated.

The assay uses a 100 µl aliquot of sample per well paired with a biotinylated detection antibody and streptavidin-HRP amplification, with a calibration curve established using the kit's recombinant IL-17A/F standard to support interpolation across the dynamic range documented in the lot-specific certificate of analysis. Reconstituted standard should be aliquoted promptly and held frozen at -20 °C to limit freeze-thaw degradation, while working dilutions and prepared wells remain stable when refrigerated.

Intended for research use only, this 96-test configuration includes a pre-coated plate frame, lyophilized recombinant IL-17A/F standard, detection reagent, substrate, and stop solution, supporting multiplexed replicate measurements in inflammation, vaccine adjuvant, and tumor microenvironment studies.