Human IL-17A ELISA Kit

The Antibodies.com Human IL-17A ELISA Kit is a quantitative sandwich immunoassay for quantifying interleukin-17A in cell culture supernatants and similar research samples. IL-17A is a pro-inflammatory cytokine produced primarily by Th17 cells and plays a central role in autoimmune pathogenesis, mucosal immunity, and host defense against extracellular bacteria and fungi.

This 96T kit uses a pre-coated monoclonal capture antibody paired with a biotinylated detection reagent, and results are read on a standard microplate reader. The detection range spans 31.25 to 2000 pg/mL with sensitivity below 9.4 pg/mL, requiring 100 uL of sample per well. No significant cross-reactivity is observed with related cytokines such as IL-17F. Reagents are supplied ready-to-use, and the kit should be stored refrigerated to preserve antibody activity through the stated shelf life.

Common research applications include cytokine profiling in autoimmune disease models, inflammatory bowel disease studies, psoriasis research, rheumatoid arthritis investigation, and vaccine adjuvant development. Investigators studying Th17 differentiation and downstream neutrophil recruitment rely on this assay to quantify IL-17A secretion under varying stimulation conditions.