Human Hyaluronidase ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Hyaluronidase in serum, plasma, tissue homogenates or other biological fluids. The assay employs a 96-well microplate pre-coated with antibody specific for Hyaluronidase (12 x 8 well strips), with a colourmetric readout at 450 nm using TMB substrate. Standards and samples are captured by the immobilised antibody, detected with a Biotinylated Anti-Hyaluronidase Antibody followed by an HRP-Avidin conjugate, and the resulting colour intensity is proportional to the amount of Hyaluronidase captured in each well.

Reported sensitivity is <1.28 ng/ml and the assay range is 3.12–200 ng/ml. Total assay time is 4 hours 30 minutes. The kit includes a pre-coated 96 well microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers; storage temperatures vary by component between +4°C and -20°C.

Spike-and-recovery performance has been evaluated in serum (average 96%, range 90–102%, n=5), EDTA plasma (average 95%, range 90–101%, n=5) and heparin plasma (average 94%, range 91–98%, n=5). Linearity of dilution has also been assessed at 1:2, 1:4, 1:8 and 1:16 dilutions across the same sample types.