Human Hyaluronan Synthase 2 ELISA Kit

From Antibodies.com, this assay is a sandwich ELISA designed for the in vitro quantitative determination of human Hyaluronan Synthase 2 in serum, plasma, tissue homogenates or other biological fluids. The kit employs a pre-coated microplate format and uses a biotinylated anti-Hyaluronan Synthase 2 detection antibody with an HRP-avidin conjugate.

The enzymatic reaction is visualised using TMB substrate, producing a blue product that turns yellow upon addition of stop solution; absorbance is then read at 450 nm and the analyte concentration calculated against a standard curve. The stated product range is 0.312–20 ng/ml with a sensitivity of <0.115 ng/ml. Total assay time is 4h 30m, and detection is colourimetric on a microplate reader. Kit components are stored at +4°C or −20°C as instructed.

Reported sample recovery averages include serum at 91% (range 80–102%, n=5), EDTA plasma at 90% (range 81–100%, n=5) and heparin plasma at 84% (range 80–89%, n=5). Dilution linearity is documented for serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16.