Human Histidine Rich Glycoprotein ELISA Kit

This Antibodies.com assay is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) intended for the in vitro quantitative determination of human Histidine Rich Glycoprotein (HRG) in serum, plasma or other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with detection performed on a colorimetric microplate reader at 450 nm. The stated sensitivity is <6.8 pg/ml and the assay range is 15.6-1000 pg/ml. Total assay time is 4 hours 30 minutes. The principle relies on an immobilised anti-HRG capture antibody, followed by a biotinylated anti-HRG detection antibody and HRP-Avidin conjugate, with TMB substrate producing a yellow product proportional to HRG concentration.

Reported recovery data from the manufacturer include average values for serum (mean 91%, individual range 80%-102%, n=5), EDTA plasma (mean 90%, range 81%-100%, n=5) and heparin plasma (mean 84%, range 80%-89%, n=5). Dilution linearity is provided for serum, EDTA plasma and heparin plasma at 1:2, 1:4, 1:8 and 1:16 dilutions. Kit components include a lyophilised standard, Detection Solution A, Detection Solution B, Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, with storage specified between +4°C and -20°C depending on the component.