Human Hepatocyte Nuclear Factor 1 Beta ELISA Kit

Antibodies.com offers a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of human HNF-1B (Hepatocyte Nuclear Factor 1 Beta) in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate and a biotinylated anti-HNF-1B detection antibody, with an HRP-Avidin conjugate and TMB substrate used to generate a colour change that is read at 450nm in a microplate reader.

Quantification is supported over a working range of 0.156–10 ng/ml with a sensitivity of 0.047 ng/ml, and the full protocol can be completed in approximately 4 hours 30 minutes. Reagents supplied include lyophilised standard, Detection Solutions A and B, Wash Buffer (30X concentrate), Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers, to be stored at +4°C or −20°C as directed.

Spike-and-recovery evaluation (n=5) gave mean recoveries of 91% in serum (range 83–100%), 96% in EDTA plasma (range 90–103%), and 95% in heparin plasma (range 89–102%). Linearity across serial dilutions (1:2 to 1:16) returned serum recoveries of 83–110%, 79–100%, 90–98%, and 82–108%; EDTA plasma recoveries of 90–98%, 90–105%, 73–109%, and 87–91%; and heparin plasma recoveries of 88–103%, 77–105%, 95–98%, and 87–110%.