Human Hepatitis A Virus Cellular Receptor 2 ELISA Kit

This sandwich ELISA kit from Antibodies.com enables quantitative in vitro determination of Hepatitis A Virus Cellular Receptor 2 (TIM-3) in tissue homogenates, cell lysates, and other biological fluids from human samples. The assay uses a pre-coated 96-well microplate platform (12 x 8 well strips) with a biotinylated detection antibody and HRP-avidin conjugate; the enzymatic reaction is visualised with TMB substrate and read at 450 nm on a colorimetric microplate reader. Quantitative range is 78.1-5000 pg/ml with a sensitivity below 36 pg/ml, and the full protocol takes approximately 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed. Reported average recovery values are 91% in serum, 91% in EDTA plasma, and 84% in heparin plasma (n=5), with dilution linearity demonstrated from 1:2 to 1:16 across these matrices.