Human Hemojuvelin ELISA Kit

Antibodies.com supplies this Human Hemojuvelin ELISA Kit, a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Hemojuvelin. The assay is reactive with human samples and is suitable for serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Reported performance includes a product range of 0.156–10 ng/ml and a sensitivity of <0.062 ng/ml. The assay is performed on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a total run time of 4h 30m. Detection is colorimetric, read at 450 nm in a microplate reader, using a biotinylated anti-Hemojuvelin antibody and an HRP-Avidin conjugate with TMB substrate; the concentration of Hemojuvelin is calculated by reference to a standard curve.

Validated recovery ranges include serum 80%–102% (average 91%), EDTA plasma 81%–100% (average 90%) and heparin plasma 80%–89% (average 84%). Kit components are stored at +4°C or -20°C as instructed and the kit should not be used past the stated expiration date.