Human Heat Shock Protein 90kDa Alpha A1 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit is designed for the in vitro quantitative determination of human HSP90 alpha in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids [S1]. The assay uses a pre-coated microplate platform with colourmetric detection read on a microplate reader at 450 nm, and the total assay time is 4h 30m [S1].

Reported performance figures include a product range of 1.56–100 ng/ml and a sensitivity of <0.55 ng/ml [S1]. Reactivity is specific to human samples [C0, S1]. Spike-and-recovery testing reported average recoveries of 91% for serum (range 80–102%), 91% for EDTA plasma (range 81–101%), and 84% for heparin plasma (range 80–89%); linearity on dilution (1:2, 1:4, 1:8, 1:16) gave recoveries of 87–107% for serum, 84–108% for EDTA plasma, and 82–105% for heparin plasma [S1].

Kit components include a pre-coated microplate, lyophilised standard (2 vials), Detection Solution A and B (120 µl each), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml), and adhesive plate sealers, with components stored at +4°C or -20°C as instructed [S1].