Human Growth Arrest And DNA Damage Inducible Protein Alpha ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay is designed for the in vitro quantitative determination of human GADD45A in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate format with a biotinylated anti-GADD45A detection antibody and HRP-avidin conjugate. Signal is developed using TMB substrate and read at 450 nm on a colorimetric microplate reader. Total assay time is 4h 30m, and kit components are stored at +4°C or -20°C as instructed.

Reported performance characteristics include a sensitivity of <0.064 ng/ml and a quantitative range of 0.156-10 ng/ml. In validation data supplied with the kit, average recovery values of 91% (serum), 95% (EDTA plasma), and 96% (heparin plasma) are given for neat samples, with broadly comparable performance across serial dilutions from 1:2 through 1:16.