Human Glypican 4 ELISA Kit

From Antibodies.com, this Human Glypican 4 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of human Glypican 4 in serum, plasma, tissue homogenates, and other biological fluids. The assay runs on a pre-coated 96-well microplate configured as 12 x 8 well strips, with colorimetric detection read at 450 nm in a standard microplate reader.

Quantitative performance includes a measurement range of 0.156–10 ng/ml and a sensitivity of <0.051 ng/ml, with a total assay time of 4 hours 30 minutes. Spike-and-recovery testing (n=5) returned average recoveries of 91% in serum (range 80–102%), 90% in EDTA plasma (range 81–100%), and 84% in heparin plasma (range 80–89%). Dilution linearity has been assessed at 1:2, 1:4, 1:8, and 1:16 across serum, EDTA plasma, and heparin plasma samples. The workflow uses an HRP-Avidin conjugate with TMB substrate, producing a blue-coloured product that changes to yellow after addition of the acidic stop solution; colour intensity is directly proportional to Glypican 4 concentration and is quantitated against a standard curve.

Kit components are stored at +4°C or -20°C as instructed and should not be used past the expiration date. Supplied items include the pre-coated 96-well microplate, lyophilised standard (2 vials), Detection Solution A (120 µl), Detection Solution B (120 µl), wash buffer (30X, 20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml), and plate sealers (5 adhesive strips). The kit is reactive with human samples and is provided for sandwich ELISA applications targeting Glypican 4.