Human Glypican 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Glypican 1 (GPC1) in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a biotinylated anti-GPC1 detection antibody and an HRP-avidin conjugate. Signal is generated using TMB substrate, producing a blue product that turns yellow upon addition of the acidic stop solution; absorbance is read at 450 nm on a colorimetric microplate reader.

Manufacturer-reported performance includes a sensitivity of 29 pg/ml and an assay range of 78.1-5000 pg/ml. Recovery has been validated across matrices: serum 80%-102% (n=5, average 91%), EDTA plasma 81%-100% (n=5, average 90%), and heparin plasma 80%-89% (n=5, average 84%). Dilution linearity across 1:2 to 1:16 is reported for each sample type. Total assay time is 4h 30m.

Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.