Human Glycyl tRNA Synthetase ELISA Kit

The Antibodies.com Human Glycyl tRNA Synthetase ELISA Kit is a sandwich ELISA (sELISA) intended for the in vitro quantitative determination of human GARS in serum, plasma, tissue homogenates and other biological fluids.

The assay uses the sandwich enzyme immunoassay technique, with an antibody specific for GARS pre-coated onto a 96-well microplate. Standards and test samples are added to the wells, where any GARS present binds to the immobilised antibody. After incubation and washing, a Biotinylated Anti-GARS Antibody is added, followed by an HRP-Avidin conjugate. A TMB substrate solution is then used to visualise the HRP enzymatic reaction, producing a blue product that turns yellow on addition of acidic stop solution. The intensity of yellow is proportional to the amount of GARS captured in each well, and the GARS concentration is calculated by reading the O.D. absorbance at 450 nm in a microplate reader against a standard curve.

The assay has a measuring range of 0.312–20 ng/ml and a sensitivity of <0.124 ng/ml, with a total assay time of 4h 30m. The pre-coated microplate platform is supplied as 12 x 8 well strips, and components are stored at +4°C or -20°C as instructed. The kit is for use with a colorimetric microplate reader. Validation reported the following recoveries: serum (n=5) 80%–102%, average 91%; EDTA plasma (n=5) 81%–100%, average 90%; heparin plasma (n=5) 80%–89%, average 84%. Linearity was assessed at dilutions 1:2, 1:4, 1:8 and 1:16 — serum (n=5) gave 87–91%, 87–107%, 74–101% and 92–97%; EDTA plasma (n=5) gave 90–105%, 84–101%, 90–101% and 79–108%; heparin plasma (n=5) gave 84–95%, 92–105%, 82–105% and 89–91%.