Human Glycosylphosphatidylinositol Anchored Molecule Like Protein ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a total run time of 4 hours 30 minutes.

Quantitative readout is achieved by measuring absorbance at 450 nm in a colorimetric microplate reader following TMB substrate conversion, producing a yellow product whose intensity is proportional to GML concentration. The standard curve covers 0.156–10 ng/ml with a sensitivity of <0.055 ng/ml. Reagents supplied include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, stored at +4°C or –20°C as indicated per component.

Validation data report average spike-and-recovery values of 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5). Dilution linearity was demonstrated across 1:2, 1:4, 1:8, and 1:16 dilutions for all three sample types, with serum ranging 74–107%, EDTA plasma ranging 79–108%, and heparin plasma ranging 82–105%.