Human Glycoprotein 39, Cartilage ELISA Kit

A sandwich ELISA kit from Antibodies.com, catalogued under the target name Glycoprotein 39, Cartilage, for the quantitative in vitro measurement of YKL-40/CHI3L1 in human plasma. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a biotinylated anti-YKL-40/CHI3L1 detection antibody, HRP-avidin conjugate and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader.

Manufacturer specifications state a sensitivity of <12.2 pg/ml and a measurable range of 31.2-2000 pg/ml. The total assay time is 4h 30m and kit components are stored at +4°C or -20°C as instructed. Reported average recovery in spiked samples was 91% (serum, n=5, range 83-100%), 94% (EDTA plasma, n=5, range 91-98%) and 94% (heparin plasma, n=5, range 90-99%).

Linearity of dilution was assessed at 1:2, 1:4, 1:8 and 1:16 across serum, EDTA plasma and heparin plasma (n=5 each), with reported recovery ranges of 78-102% / 90-100% / 80-104% / 86-97% (serum), 95-103% / 81-109% / 87-91% / 88-107% (EDTA plasma) and 74-101% / 92-97% / 90-105% / 84-102% (heparin plasma).