Human Glutathione Synthetase ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of human Glutathione Synthetase in serum, plasma, tissue homogenates, and other biological fluids. The assay uses a pre-coated 96-well microplate (12 × 8 well strips) and follows a standard sandwich enzyme immunoassay workflow with a biotinylated detection antibody, HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm in a microplate reader. The kit is for research use only and is not intended for diagnostic or therapeutic use.

Performance specifications include a sensitivity of 0.127 ng/ml and a standard curve range of 0.312–20 ng/ml, with a typical total assay time of approximately 4 hours 30 minutes. Kit components are stored at +4 °C or −20 °C as instructed. Reported spike-and-recovery values averaged 91% in serum (range 80–102%, n=5), 90% in EDTA plasma (range 81–100%, n=5), and 84% in heparin plasma (range 80–89%, n=5).