Human Glutathione S Transferase Pi ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Glutathione S Transferase Pi (GST3/GST pi) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay uses a biotinylated detection antibody and HRP-avidin conjugate on a pre-coated microplate (12 x 8 well strips), with TMB substrate read colorimetrically at 450 nm. Performance specifications include a product range of 1.56-100 ng/ml, a sensitivity of <0.66 ng/ml, and a total assay time of 4h 30m.

Reported recovery across sample matrices (n=5) averages 91% in serum (range 80-102%), 90% in EDTA plasma (range 81-100%), and 84% in heparin plasma (range 80-89%). Dilution linearity has been demonstrated at 1:2, 1:4, 1:8, and 1:16 across the same matrices. Kit components should be stored at +4°C or -20°C as instructed, and the product should not be used past its expiration date.