Human Glutamate Receptor, Ionotropic, AMPA 3 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human Glutamate Receptor 3/GluA3 in tissue homogenates and other biological fluids. The assay employs a pre-coated 96-well microplate format with a biotinylated detection antibody and an HRP-avidin conjugate, producing a colourimetric signal read at 450 nm via TMB substrate. Reported total assay time is 4 h 30 min.

Quoted performance specifications include a sensitivity of <0.116 ng/ml and an assay range of 0.312–20 ng/ml. The kit is intended for use with a colourimetric microplate reader, and components are stored at +4 °C or −20 °C as instructed; the product should not be used past its expiration date.

Manufacturer recovery data (n = 5) report averages of 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%), and 84% for heparin plasma (range 80–89%), with dilution linearity also evaluated across 1:2 to 1:16 dilutions.