Human Gigaxonin ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) configured for the in vitro quantitative determination of human Gigaxonin in tissue homogenates and other biological fluids. The kit employs a pre-coated 96-well microplate (12 x 8 well strips) together with a biotinylated anti-Gigaxonin detection antibody and an HRP-avidin conjugate, and the enzymatic reaction is visualised with TMB substrate and read as optical density at 450 nm.

Quantitative performance reported for the kit includes an assay range of 0.156–10 ng/ml and a sensitivity of <0.055 ng/ml, with a colourimetric microplate reader as the required instrument. Total assay time is stated as 4 h 30 m, and kit components should be stored at +4 °C or −20 °C as instructed.

Sample validation figures supplied with the kit show average recovery of 91% in serum (range 80%–102%), 90% in EDTA plasma (81%–100%) and 84% in heparin plasma (80%–89%). The product is intended for research use only and is not for diagnostic or therapeutic applications.