Human Gap Junction Protein Beta 1 ELISA Kit

A sandwich ELISA kit from Antibodies.com designed for the in vitro quantitative determination of human Connexin 32/GJB1 (Gap Junction Protein Beta 1) in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate format with colorimetric detection read at 450 nm on a microplate reader.

According to the manufacturer, the assay range is 0.312-20 ng/ml with a sensitivity of 0.108 ng/ml, and the total assay time is 4 hours 30 minutes. Reported average recovery values are 91% for serum (range 83%-99%, n=5), 89% for EDTA plasma (range 80%-99%, n=5), and 90% for heparin plasma (range 82%-99%, n=5). Linearity has been assessed at 1:2, 1:4, 1:8 and 1:16 dilutions, with reported ranges of 87-109%, 74-104%, 90-94% and 86-108% for serum; 86-101%, 90-101%, 78-110% and 84-96% for EDTA plasma; and 90-101%, 77-107%, 93-96% and 89-109% for heparin plasma. Kit components are stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.