Human Galactosidase Beta ELISA Kit

This Antibodies.com sandwich enzyme-linked immunosorbent assay (sELISA) is designed for the in vitro quantitative determination of human GLB1/beta Galactosidase in serum, plasma, tissue homogenates, cell lysates or other biological fluids. The kit is supplied as a pre-coated 96-well microplate (12 x 8 well strips) on which an antibody specific for GLB1/beta Galactosidase is immobilised. Standards and test samples are added to the wells and any target analyte present is captured by the immobilised antibody. After incubation and washing, a Biotinylated Anti-GLB1/beta Galactosidase Antibody is added, followed by HRP-Avidin conjugate and TMB substrate. The blue product of the enzymatic reaction turns yellow on addition of acidic stop solution, with the density of yellow being proportional to the amount of analyte captured in each well. The concentration is calculated from the absorbance read at 450 nm in a microplate reader against the standard curve.

Key specifications include a quantitative sandwich format with colourmetric detection, a measuring range of 0.312–20 ng/ml, a sensitivity of 0.105 ng/ml and a total assay time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed and should not be used past the stated expiration date.

Reported average recovery values across sample types were 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5) and 84% for heparin plasma (range 80–89%, n=5). Dilution linearity data are also reported for 1:2, 1:4, 1:8 and 1:16 dilutions across serum, EDTA plasma and heparin plasma matrices.