Human GA Binding Protein Transcription Factor Alpha ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human GABPA (GA Binding Protein Transcription Factor Alpha) in tissue homogenates and other biological fluids. The assay uses a pre-coated microplate and employs a biotinylated detection antibody with HRP-avidin and TMB substrate to generate a colourmetric signal read at 450 nm.

Reported performance specifications include a sensitivity of 0.057 ng/ml and an assay range of 0.156-10 ng/ml. The total assay time is 4h 30m, and the kit is intended for use with a colorimetric microplate reader. Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.

Sample recovery data supplied with the product show average values of 91% in serum (range 80%-102%, n=5), 90% in EDTA plasma (range 81%-100%, n=5), and 84% in heparin plasma (range 80%-89%, n=5). Linearity of dilution has been demonstrated across 1:2 to 1:16 dilutions in the same matrices.