Human G Protein Coupled Receptor 109B ELISA Kit

A sandwich enzyme-linked immunosorbent assay (ELISA) from Antibodies.com is designed for the in vitro quantitative determination of human G Protein Coupled Receptor 109B (HCAR3) in tissue homogenates, cell lysates, or other biological fluids. The kit employs a pre-coated 96-well microplate, with an antibody specific for HCAR3 immobilised on the plate surface.

The quantitative sandwich immunoassay uses a Biotinylated Anti-HCAR3 Antibody and an HRP-Avidin conjugate for detection, with a TMB substrate solution that produces a yellow colour proportional to the amount of HCAR3 captured in each well. The concentration of HCAR3 is calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve. Total assay time is 4h 30m, and components are stored at +4°C or -20°C as instructed.

The assay has a sensitivity of <0.061 ng/ml and a working range of 0.156-10 ng/ml. Reported recovery values are 84-102% (average 93%) for serum, 90-102% (average 96%) for EDTA plasma, and 92-98% (average 95%) for heparin plasma, with linearity data provided across 1:2 to 1:16 dilutions for serum, EDTA plasma, and heparin plasma.