Human Fibroblast Growth Factor 7 ELISA Kit

Manufactured for Antibodies.com, this sandwich ELISA kit enables in vitro quantitative determination of human KGF/FGF7 (Fibroblast Growth Factor 7) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids [S1].

The assay uses a 96-well microtiter plate pre-coated with an antibody specific for KGF/FGF7. Standards and samples are added to the wells, followed by Biotinylated Anti-KGF/FGF7 Antibody and an HRP-Avidin conjugate. A TMB substrate is then added and the reaction is stopped with acidic stop solution; absorbance is read at 450 nm on a colorimetric microplate reader [S1].

Key performance characteristics quoted by the manufacturer include a measuring range of 15.6–1000 pg/ml, a sensitivity of 5.2 pg/ml, and an assay time of 4 h 30 min [S1]. Reported recovery values are 80–102% (average 91%) for serum, 81–100% (average 90%) for EDTA plasma, and 80–89% (average 84%) for heparin plasma (n=5 each) [S1]. Dilution linearity at 1:2, 1:4, 1:8 and 1:16 is reported across serum (87–91%, 87–107%, 74–101%, 92–97%), EDTA plasma (90–105%, 84–101%, 90–101%, 79–108%) and heparin plasma (84–95%, 92–105%, 82–105%, 89–91%) (n=5 each) [S1]. Kit components include a pre-coated microplate strip, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers; components should be stored at +4°C or -20°C as instructed [S1].