Human Fibroblast Growth Factor 13 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Fibroblast Growth Factor 13 (FGF13) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate arranged as 12 x 8 well strips.

An antibody specific for FGF13 is immobilized on the plate to capture the target from standards and test samples. After incubation and washing, a Biotinylated Anti-FGF13 Antibody binds the captured FGF13, followed by addition of an HRP-Avidin conjugate. TMB substrate is added to visualize the reaction, producing a blue colour that changes to yellow upon addition of acidic stop solution. Absorbance is read at 450 nm on a colorimetric microplate reader and the FGF13 concentration is calculated against the standard curve.

The kit provides a quantitative measurement range of 15.6-1000 pg/ml with a sensitivity of <5.4 pg/ml and a total assay time of 4 hours 30 minutes. Recovery has been demonstrated in serum (average 91%, range 83-100%), EDTA plasma (average 96%, range 90-103%) and heparin plasma (average 95%, range 89-102%), with linearity shown across dilutions from 1:2 to 1:16. Components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage at +4°C or -20°C as instructed.