Human Fibrinogen Beta ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Fibrinogen beta Chain in plasma samples. The assay is configured as a sandwich ELISA on a pre-coated 96-well microplate (12 x 8 well strips), with colorimetric detection read at 450 nm in a microplate reader and quantification against a standard curve.

Reported performance characteristics include a product range of 78.1-5000 ng/ml, a sensitivity of <29 ng/ml, and an assay time of 4h 30m. Spiking recovery across five replicates has been reported at 80-102% (average 91%) for serum, 81-100% (average 90%) for EDTA plasma, and 80-89% (average 84%) for heparin plasma, with dilution linearity ranges provided across 1:2, 1:4, 1:8 and 1:16 sample dilutions.

Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.