Human Fetuin A ELISA Kit

A sandwich-format enzyme-linked immunosorbent assay designed for the quantitative in vitro determination of human Fetuin A. Intended for use with cell culture supernatant, serum, and plasma samples, the assay is built on a quantitative sandwich ELISA principle in which a monoclonal antibody specific for human Fetuin-A is immobilized on microwell plates. Standards or samples are added, followed by a biotin-linked monoclonal antibody specific for Fetuin-A; after washing to remove unbound material, streptavidin-HRP is introduced and a substrate solution reacts with HRP to develop colour in proportion to the amount of Fetuin-A bound. The reaction is stopped by addition of acid and the optical density is measured with a microplate reader.

The kit, supplied by Antibodies.com, shows no significant cross-reactivity and can assay both native and recombinant human Fetuin-A. Reported sensitivity is <2.22 pg/ml and the assay range is 46.88 - 3000 pg/ml. Detection is colorimetric on a microplate platform, and components should be stored at +4°C and not used past the expiration date.