Human Ferritin, Light Polypeptide ELISA Kit

From Antibodies.com, this Human Ferritin Light Chain ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Ferritin Light Chain in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

The assay employs the sandwich enzyme immunoassay technique. An antibody specific for Ferritin Light Chain is pre-coated onto a microplate. Standards and test samples are added to the wells, where Ferritin Light Chain present in the sample binds to the immobilized antibody. Following incubation and washing, a Biotinylated Anti-Ferritin Light Chain Antibody is added, followed by an HRP-Avidin conjugate. TMB substrate solution is then used to visualise the HRP enzymatic reaction, producing a blue product that changes to yellow upon addition of acidic stop solution. The density of yellow is proportional to the amount of Ferritin Light Chain captured in each well. The concentration of Ferritin Light Chain can be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Key specifications include a product range of 31.2-2000 pg/ml, a sensitivity of 14.9 pg/ml, an assay time of 4h 30m, and colourimetric detection. Components should be stored at +4°C or -20°C as instructed. Reported recovery averages are 91% for serum (range 80%-102%, n=5), 90% for EDTA plasma (range 81%-100%, n=5), and 84% for Heparin plasma (range 80%-89%, n=5).