Human Fatty Acid Transport Protein 5 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is intended for the quantitative determination of human Fatty Acid Transport Protein 5 in tissue homogenates or other biological fluids. The procedure uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with results read colourimetrically at 450 nm in a microplate reader. The kit's product range is quoted as 0.156-10 ng/ml and sensitivity as <0.055 ng/ml, with a total assay time of 4 hours 30 minutes.

The manufacturer's validation summary reports mean recovery of 91% in serum (range 80-102%, n=5), 90% in EDTA plasma (range 81-100%, n=5), and 84% in heparin plasma (range 80-89%, n=5), alongside linearity data for serial dilutions at 1:2, 1:4, 1:8, and 1:16 across the same sample types. Components supplied include the pre-coated plate, lyophilised standard, detection solutions A and B, a 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage conditions specified per component at +4°C or -20°C.