Human Fatty Acid Binding Protein 2, Intestinal ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human Intestinal Fatty Acid Binding Protein (I-FABP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay uses a pre-coated 96-well microplate, a biotinylated detection antibody with HRP-Avidin conjugate, TMB substrate, and absorbance read at 450 nm. Total assay time is 4 hours 30 minutes, and the result is captured on a colorimetric microplate reader.

Reported performance characteristics include a measuring range of 0.156–10 ng/ml and a sensitivity of 0.057 ng/ml. Spike-and-recovery values across sample types are reported, with average recoveries of 91% in serum (range 80%–102%), 90% in EDTA plasma (range 81%–100%), and 84% in heparin plasma (range 80%–89%). Kit components should be stored at +4°C or –20°C as instructed, and the assay must not be used past its expiration date.