Human Fatty Acid Binding Protein 1, Liver ELISA Kit

Supplied under the Antibodies.com brand, this sandwich Enzyme-Linked Immunosorbent Assay kit is designed for the in vitro quantitative determination of human Liver Fatty Acid Binding Protein 1 (Liver FABP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The format uses a pre-coated microplate; bound analyte is detected with a biotinylated anti-Liver FABP antibody, HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm in a microplate reader.

According to the manufacturer's product information, the stated detection range is 1.56–100 ng/ml and sensitivity is 0.54 ng/ml, with a total assay time of 4 hours 30 minutes. Reported spike-and-recovery values are 80%–102% (average 91%) in serum, 81%–100% (average 90%) in EDTA plasma, and 80%–89% (average 84%) in heparin plasma, each measured at n=5.

Reagents are stored at +4°C or -20°C depending on the component, and the kit should not be used past its expiration date.