Human Family With Sequence Similarity 20, Member A ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) kit from Antibodies.com, intended for the in vitro quantitative determination of human FAM20A in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate with Biotinylated Anti-FAM20A Antibody, HRP-Avidin conjugate and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader. Reported sensitivity is <0.069 ng/ml and the assay range is 0.156–10 ng/ml. Total assay time is 4h 30m.

In addition to tissue homogenates, the manufacturer reports validation data for serum, EDTA plasma and heparin plasma. Reported recovery averages are 91% for serum (range 83–100%, n=5), 97% for EDTA plasma (range 91–103%, n=5) and 95% for heparin plasma (range 89–102%, n=5). Linearity-of-dilution data across 1:2 to 1:16 dilutions are also provided. Kit components are stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.