Human Extracellular Matrix Protein 1 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the quantitative in vitro determination of human Extracellular Matrix Protein 1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

The assay uses a pre-coated microplate platform and a biotinylated antibody/HRP-avidin detection workflow with TMB substrate, producing a colourmetric signal read at 450 nm. It covers a measuring range of 31.2–2000 pg/ml with a sensitivity of <13.2 pg/ml, and the full protocol is completed in 4h 30m. Reported average recovery is 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma, with linearity demonstrated across dilutions from 1:2 to 1:16. Kit components are stored at +4°C or -20°C as instructed and the kit must not be used past its expiration date.