Human Epithelial Stromal Interaction 1, Breast ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human EPSTI1 in tissue homogenates, cell lysates or other biological fluids. Reactivity: Human. Detection is colorimetric, with absorbance read at 450 nm in a microplate reader.

The assay uses a pre-coated 96-well microplate (12 x 8 well strips) bearing an antibody specific for EPSTI1, followed by a Biotinylated Anti-EPSTI1 Antibody, HRP-Avidin conjugate and TMB substrate. Total assay time is 4h 30m. The stated product range is 0.312-20 ng/ml with a sensitivity of 0.113 ng/ml. Kit components include a lyophilized standard, Detection Solution A, Detection Solution B, Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, to be stored at +4°C or -20°C as instructed.

Reported validation data: average recovery in serum (n=5) was 91% (range 80%-102%), in EDTA plasma (n=5) 91% (range 81%-101%), and in heparin plasma (n=5) 84% (range 80%-89%). Linearity was demonstrated at 1:2, 1:4, 1:8 and 1:16 dilutions across serum (87-91%, 87-107%, 74-101%, 92-97%), EDTA plasma (90-105%, 84-101%, 90-101%, 79-108%) and heparin plasma (84-95%, 92-105%, 82-105%, 89-91%).