Human Ephrin Type A Receptor 1 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Eph Receptor A1/EphA1 in tissue homogenates, cell lysates or other biological fluids. The assay uses the sandwich enzyme immunoassay technique, in which an antibody specific for Eph Receptor A1/EphA1 is pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells, allowing the target to be captured by the immobilized antibody. Following washing, a Biotinylated Anti-Eph Receptor A1/EphA1 Antibody is added, followed by an HRP-Avidin conjugate. TMB substrate solution produces a blue colour that turns yellow upon addition of acidic stop solution; the density of yellow is proportional to the amount of target captured, and the concentration is calculated by reading the O.D. absorbance at 450 nm in a microplate reader against a standard curve.

The kit has a reported sensitivity of 0.124 ng/ml and an assay range of 0.312–20 ng/ml, with a total assay time of 4 h 30 min. It is intended for use with a colorimetric microplate reader. Reported average recovery values are 91% in serum, 90% in EDTA plasma, and 84% in heparin plasma (n=5). Across dilutions from 1:2 to 1:16, reported recovery ranges are 87–107% in serum, 79–108% in EDTA plasma, and 82–105% in heparin plasma. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.