Human Ephrin A5 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is intended for the in vitro quantitative determination of human Ephrin A5 in serum, plasma, tissue homogenates and other biological fluids. The assay is performed on a pre-coated 96-well microplate configured as 12 x 8 well strips. Standards and test samples are added to the wells, with target captured by the immobilized antibody. After washing, a biotinylated detection antibody binds the captured analyte, followed by addition of an HRP-avidin conjugate. A TMB substrate solution produces a blue-coloured product that changes to yellow upon addition of an acidic stop solution, and the absorbance is read at 450 nm on a colorimetric microplate reader. The reported assay range is 0.156-10 ng/ml, with a sensitivity of <0.059 ng/ml. Total assay time is 4h 30m, and kit components should be stored at +4°C or -20°C as instructed.