Human Endothelin Receptor B ELISA Kit

From Antibodies.com, this sandwich ELISA kit is intended for the in vitro quantitative determination of human Endothelin B Receptor/ET-B in tissue homogenates, cell lysates, and other biological fluids. The assay uses a 96-well microplate pre-coated with a capture antibody, a biotinylated anti-ET-B detection antibody, an HRP-avidin conjugate, and a TMB substrate; absorbance is measured at 450 nm in a colorimetric microplate reader.

The manufacturer states a product range of 0.312–20 ng/ml and a sensitivity of <0.113 ng/ml, with a total assay time of 4 h 30 min. Reported average recovery values are 91% in serum (range 80%–102%, n=5), 90% in EDTA plasma (range 81%–100%, n=5), and 84% in heparin plasma (range 80%–89%, n=5). Linearity data covering dilutions from 1:2 to 1:16 are provided for serum, EDTA plasma, and heparin plasma, and the kit is stated to be suitable for cell lysates and tissue homogenates.