Human Endothelial Cell Adhesion Molecule ELISA Kit

Designed for the in vitro quantitative determination of human ESAM (Endothelial Cell Adhesion Molecule), this sandwich ELISA from Antibodies.com supports quantitative measurement of the target in tissue homogenates, cell lysates, serum, EDTA plasma and heparin plasma. The kit employs a pre-coated microplate format with biotinylated anti-ESAM antibody and HRP-avidin conjugate chemistry, producing a colorimetric signal via TMB substrate that is read at 450 nm.

Manufacturer-reported performance includes an assay range of 0.312–20 ng/ml and a sensitivity of <0.117 ng/ml, with a total assay time of 4h 30m. Sample recovery testing yielded averages of 91% for serum (range 80%–102%, n=5), 90% for EDTA plasma (range 81%–100%, n=5), and 84% for heparin plasma (range 80%–89%, n=5). Kit components should be stored at +4°C or -20°C as instructed.